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KMID : 0380020120270050295
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Korean Journal of Biotechnology and Bioengineering
2012 Volume.27 No. 5 p.295 ~ p.300
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In vivo Dendritic Cell Migration Tracking Using Near-infrared (NIR) Imaging
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Lee Jun-Ho
Jung Nam-Chul
Lee Eun-Gae
Lim Dae-Seog
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Abstract
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Matured dendritic cells (DCs) begin migration with their release from the bone marrow (BM) into the blood and subsequent traffic into peripheral lymphoid and non-lymphoid tissues. Throughout this long movement, migrating DCs must apply specialized skills to reach their target destination. Non-invasive in vivo cell-tracking techniques are necessary to advance immune cell-based therapies. In this study, we used a DiD cell-tracking solution for in vivo dendritic cell tracking in naive mice. We tracked DiD (non-invasive fluorescence dye)-labeled mature dendritic cells using the Near Infrared (NIR) imaging system in normal mice. We examined the immunophenotype of DiD-labeled cells compared with non-labelled mature DCs, and obtained time-serial images of NIR-DC trafficking after mouse footpad injection. In conclusion, we confirmed that DiD-labeled DCs migrated into the popliteal lymph node 24 h after the footpad injection. Here, these data suggested that the cell tracking system with the stable fluorescence dye DiD was useful as a cell tracking tool to advance dendritic cell-based immunotherapy.
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KEYWORD
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Dendritic cell, DiD, NIR, in vivo cell-tracking, Immunotherapy
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